Molecular Biology Reagents and Kits
Biochain Institute Inc Total RNA - Monkey (Rhesus) Normal Tissue: Bladder, 50 ug/PK
BioChain's Total RNAs are isolated from a wide variety of documented human normal, diseased, and tumor tissues, mouse, rat, monkey, and plant tissues. Total RNA isolation is performed using proprietary techniques. Contamination by RNase, genomic DNA polysaccharides, and proteoglycans has been effectively eliminated. The integrity of the total RNAs is assured by checking for a ratio of greater than 1:1 between 28s and 18s ribosomal RNA. High efficiency reverse transcription using the Total RNA is demonstrated.
Biochain Institute Inc Total RNA - Human Adult Normal Tissue: Brain: Cerebellum (left), 10 ug/PK
BioChain's Total RNAs are isolated from a wide variety of documented human normal, diseased, and tumor tissues, mouse, rat, monkey, and plant tissues. Total RNA isolation is performed using proprietary techniques. Contamination by RNase, genomic DNA polysaccharides, and proteoglycans has been effectively eliminated. The integrity of the total RNAs is assured by checking for a ratio of greater than 1:1 between 28s and 18s ribosomal RNA. High efficiency reverse transcription using the Total RNA is demonstrated.
Bioworld RNase AWAY™ 1L
RNase Away™ removes RNase and DNA contamination from glassware and plastic quickly and economically. Simply apply RNase away to surface or soak as needed and rinse with water. Non-abrasive formulation does not contain strong acids. Effective for use with electrophoresis equipment, glass plates, and other laboratory equipment. Specification: Removes RNase and DNA - Ready to use - Non-abrasive. Molecular Weight : 18.02 g/mol, pH : 6.0 - 8.0 at 25 °C, Solubility : completely miscible, Appearance Form : Liquid, Appearance Color : Clear, Melting Point : 0.0 °C, Boiling Point : 100.0 °C, Density : 1.023 g/cm3. 1L
New England Biolabs, Inc. NEBNext Globin & rRNA Depletion Kit (Human/Mouse/Rat) – 6 reactions
The NEBNext Globin & rRNA Depletion Kit (Human/Mouse/Rat) employs the NEBNext RNase H-based RNA depletion workflow to deplete adult, embryonic and fetal globin mRNA (HBA1/2, HBB, HBD, HBM, HBG1/2, HBE1, HBQ1 and HBZ), cytoplasmic rRNA (5S, 5.8S, 18S, 28S, ITS and ETS) and mitochondrial rRNA (12S and 16S). The kit is effective with human, mouse and rat total RNA preparations, both intact and degraded. The resulting depleted RNA is suitable for RNA-seq, random-primed cDNA synthesis, or other downstream RNA analysis. This kit can also be used following poly(A) mRNA enrichment (e.g., using the NEBNext poly(A) mRNA Magnetic Isolation Module NEB #E7490), so that the final depleted RNA contains only mRNA of interest and no non-coding RNA.
New England Biolabs, Inc. NEBNext Ultra™ II RNA Library Prep with Sample Purification Beads – 24 reactions
The Ultra II RNA Library Prep Kit for Illumina is designed for non-directional (non-strand-specific) RNA library construction, and delivers significantly increased sensitivity and specificity from your RNA-seq experiments, from ever-decreasing amounts of input RNA. In conjunction with ribosomal RNA (rRNA) depletion or poly(A) mRNA enrichment, the kit enables the production of high quality libraries from 5 ng or 10 ng of Total RNA, respectively, up to 1µg. For convenience, this kit includes SPRISelect beads.
Biochain Institute Inc Total RNA - Human Adult Normal Tissue: Bone Marrow, Ambient temperature shipping, 10 ug/pk
Total RNA is isolated by modified guanidine thiocyanate techniques and stored in RNA storage buffer. The vials were then dried for room temperature shipping and storage. The total RNA need to be reconsitituted before use. Store the vials at -80 ℃ after reconstitution.
Biochain Institute Inc Total RNA - Human Adult Normal Tissue: Thyroid, 50 ug/PK
BioChain's Total RNAs are isolated from a wide variety of documented human normal, diseased, and tumor tissues, mouse, rat, monkey, and plant tissues. Total RNA isolation is performed using proprietary techniques. Contamination by RNase, genomic DNA polysaccharides, and proteoglycans has been effectively eliminated. The integrity of the total RNAs is assured by checking for a ratio of greater than 1:1 between 28s and 18s ribosomal RNA. High efficiency reverse transcription using the Total RNA is demonstrated.
Zymo Research Corporation RNA Clean & Concentrator™-5, 200 preps
This 5 minute procedure is based on the use of a unique single-buffer system and Zymo-Spin™ technologythat allows for selective recovery of total RNA (> 17nt), large RNAs (> 200 nt), and/or small RNAs (17-200 nt).The procedure is easy: Add binding buffer and ethanol to your sample, then bind, wash and elute ultra-pure RNA. The RNA can be eluted from the Zymo-Spin™ IC Column in as little as ≥ 6 µl of RNase-free water. The highly concentrated, purified RNA is suitable for all subsequent analyses and molecular manipulations.
Norgen Biotek Corp RNase-Free DNase I Kit, 50 Reactions
Norgen's RNA purification kits isolate total RNA with minimal amounts of genomic DNA contamination. However, for some sensitive downstream applications, it may be desirable to remove all traces of residual DNA. Norgen's RNase-free DNAse I Kit, with Enzyme Incubation Buffer, can be used for optional on-column DNase digestion with any of Norgen's RNA purification kits. Alternatively, after isolating total RNA using one of Norgen's RNA purification kits, the RNA elution can be treated with this DNase I. The RNA can then be purified from the DNase using Norgen's RNA Clean-Up and Concentration Kit (Cat# 23600), and the RNA can then be used in downstream applications.
Biochain Institute Inc Total RNA - Human Adult Normal Tissue: Salivary Gland, 10 ug/PK
BioChain's Total RNAs are isolated from a wide variety of documented human normal, diseased, and tumor tissues, mouse, rat, monkey, and plant tissues. Total RNA isolation is performed using proprietary techniques. Contamination by RNase, genomic DNA polysaccharides, and proteoglycans has been effectively eliminated. The integrity of the total RNAs is assured by checking for a ratio of greater than 1:1 between 28s and 18s ribosomal RNA. High efficiency reverse transcription using the Total RNA is demonstrated.
Sigma Aldrich Fine Chemicals Biosciences RNA/DNA Stabilization Reag
RNA/DNA Stabilization Reagent for Blood/Bone Marrow Instructions for Use
Research Products International Corp Direct-zol RNA MiniPrep Plus w/TRI Reagent, & Zymo-Spin IIICG Columns, Capped, 50 Preps
The Direct-zol RNA MiniPrep Plus Kits are RNA purification kits that provide a streamlined method for the purification of up to 100 µg (per prep) of high-quality RNA directly from samples in TRI Reagent® or similar. Total RNA, including small RNAs (17-200 nt), is effectively isolated from a variety of sample sources (cells, tissues, serum, plasma, blood, biological liquids, etc.). Isolation of RNA by conventional phase separation was shown to selectively enrich for some species of miRNA, leading to bias in downstream analysis. The Direct-zol method assures unbiased recovery of small RNAs including miRNA. The procedure is easy. Simply apply a prepared sample in TRI Reagent® directly to the Zymo-Spin Column and then bind, wash, and elute the RNA. No phase separation, precipitation, or post-purification steps are necessary.
Biochain Institute Inc Total RNA - Human Adult Normal Tissue: Heart: Pulmonic Valve, 10 ug/PK
BioChain's Total RNAs are isolated from a wide variety of documented human normal, diseased, and tumor tissues, mouse, rat, monkey, and plant tissues. Total RNA isolation is performed using proprietary techniques. Contamination by RNase, genomic DNA polysaccharides, and proteoglycans has been effectively eliminated. The integrity of the total RNAs is assured by checking for a ratio of greater than 1:1 between 28s and 18s ribosomal RNA. High efficiency reverse transcription using the Total RNA is demonstrated.
Zyagen Labs Human Universal Reference Total RNA extracted from wide variety of male and female HUMAN tissues. Made at industrial scales to minimize lot-to-lot variation. Ideal for microarray, NB, PCR & many other applications. 0.05mg/pK at 1mg/ml
Zyagen Universal Reference Total RNA is composed of highly pure intact total RNA isolated from wide variety of male & female tissues. Total RNA is extracted using modified guanidine thiocyanate method, treated with DNase & packed at a concentration of 1mg/ml. Features: High quality intact RNA without genomic DNA contamination. Consistent control for standard data set comparisons. Extracted from large variety of male & female tissues for broad gene coverage. Made at industrial scales to minimize lot-to-lot variation Quality Control: The integrity of RNA sample as indicated by intact ribosomal RNA is verified by agarose gel electrophoresis. The purity of RNA is assessed by NanoDrop (A260/A280: 1.9-2.1). Residual DNA contamination is tested by PCR. Applications: Reference RNA is ideal for microarray analysis, Northern blotting, ribonuclease protection assay, SI nuclease assay, RT-PCR/Q-PCR/RACE analysis, cDNA synthesis, microRNA studies, & purification of mRNA for library construction.
Biochain Institute Inc Total RNA - Human Adult Normal Tissue: Seminal Vesicle, 10 ug/PK
BioChain's Total RNAs are isolated from a wide variety of documented human normal, diseased, and tumor tissues, mouse, rat, monkey, and plant tissues. Total RNA isolation is performed using proprietary techniques. Contamination by RNase, genomic DNA polysaccharides, and proteoglycans has been effectively eliminated. The integrity of the total RNAs is assured by checking for a ratio of greater than 1:1 between 28s and 18s ribosomal RNA. High efficiency reverse transcription using the Total RNA is demonstrated.